Video lessons on EM and Cryo-EM by Prof Jensen

I present in this post my last discovery, the “Course on Cryo EM“.

It is the best one I have found in internet (Youtube) related to the topic of electron microscopy and that consists of 33 video lessons that you can follow in Youtube! You can check the contents below. Parts 1 to 3 presents concepts for understanding what is behing electron microscopes and electron microscopy. Parts 4 to 7 are focused in the application of EM to those researcher interested in cell biology.

The material is presented by Professor Grant Jensen of Caltech University who is a renowned scientist in the field of Cryo-EM and the head of the Jensen Lab at Caltech University.

The videos are carefully edited, with good sound and image quality. The presenter, Professor Jensen, explains at a good pace with an interactive screen and great clarity from the main  hardware parts of an electron microscope to the more obscure ideas behind image formation, electron phases and crystallography. The course is very complete, and it includes sample preparation of biological samples for Cryo EM. Finally, the most advanced techniques for sample characterisation in three-dimensions including single particle analysis and cryo electron tomography are described. A review on Cryo EM can be found also here in the 25th Anniversary issue of Microscopy & Analysis (September 2012) written by Julia Mahamid and Wolfgang Baumeister.

In the next video Prof Jensen explains himself the goal and contents of his course.

CONTENTS OF THE COURSE:

Part 1: Currents, coils, knobs and names – basic anatomy of the EM

  • Electron guns
  • EM lenses
  • The column, knobs and names
  • The sample chamber
  • Energy filters
  • Detectors
  • Vacuum systems
  • Summary, sfatey

Part 2: Fourier tRnasforms and reciprocal space for the beginner

  • One-dimensional waves and sums
  • One-dimensional Fourier space
  • Two-dimensional waves and images
  • Two-dimensional waves and filters
  • Three-dimensional waves and transforms
  • Convolution and cross-correlation

Part 3: Image formation

  • Amplitude and phase contrast, scattering
  • Wave propagation and phase shifts
  • The contrast transfer function
  • Defocus and its effects
  • Envelopes
  • CTF correction

Part 4: Sample prep: Methods involving freezing

  • Traditional “thin-section” EM
  • Staining/shadowing

Part 5: Tomography

  • Intro
  • Data collection and reconstruction
  • Identifying objects of interest

Part 6: Single particle analysis

  • Intro
  • Sample prep
  • Imaging
  • Reconstruction

Part 7: 2-D crystallography

  • Intro and sample prep
  • The Fourier transform of a 2-D crystal
  • Data collection and reconstruction
  • Helical tubes
  • Farewell

An example of video lesson:

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